The human endometrium undergoes extensive regeneration during each menstrual cycle, yet the cellular programmes coordinating this process remain incompletely understood. In particular, how epithelial, stromal, vascular and immune cell states change across the early, middle and late proliferative phases, and how these changes relate to the organisation of the basalis and functionalis, requires further investigation. This project aims to characterise the cellular composition and molecular changes of full-thickness human endometrium across the proliferative phase using single-nucleus RNA sequencing. Samples representing the early, middle and late proliferative phases will be profiled using the 10x Genomics Chromium Fixed RNA Profiling (Flex) platform. Full-thickness sampling will enable the study of populations from both the basalis and functionalis, including cells that may be underrepresented in superficial endometrial biopsies. We will identify major cell populations and their transcriptional states, examine phase-associated changes in gene expression and cellular composition, and investigate pathways associated with epithelial renewal, stromal remodelling, vascular development and immune regulation. Integration with our existing spatial transcriptomic reference will help place identified cell states within their anatomical context and support the investigation of potential interactions between neighbouring populations. Comparisons across phases will account for donor variability and relevant sample metadata. Computational resources are required to process sequencing data, perform quality control, integrate samples and conduct downstream statistical analyses. The project will generate a cellular reference of endometrial regeneration and prioritise candidate pathways for future functional studies. These findings will improve our understanding of normal uterine tissue renewal and provide a foundation for investigating impaired regeneration and uterine disorders.