Discrete chromatin domains emerge in parallel with zygotic genome activation (ZGA), establishing the regulatory landscape that guides subsequent developmental programs for embryonic patterning. The spatial organization of the genome is shaped by the interplay between euchromatin, heterochromatin, and boundary elements that insulate regulatory interactions. In Drosophila, the BEN‑solo proteins Insv and Elba function as chromatin insulators during the mid‑blastula transition, but how they contribute to the establishment and stabilization of chromatin domains remains unclear. In this study, we sought to define the role of BEN‑solo proteins in shaping chromatin organization during early embryogenesis. We have profiled genomic binding of these factors, along with chromatin state markers, transcriptomes in normal and mutant conditions. By integrating this data, we aim to identify novel mechanisms by which BEN-solo factors contribute to genome organization and animal development.