This project systematically evaluates how a specific epidrug alters genome-wide transcription factor (TF)–DNA interactions. We leverage a High-throughput Insertion of Tags Across the Genome (HITAG) library consisting of over 500 HEK293T clones, each engineered to express a distinct FLAG-tagged TF. This system enables the simultaneous profiling of hundreds of TFs in a single assay. By pairing in situ reverse transcription with nanoCUT&Tag on the 10x Genomics single-cell platform, we can concurrently identify the FLAG-TF and map its genomic occupancy at single-cell resolution. This unprecedented throughput allows us to uncover how chromatin-modifying drugs reshape TF binding across diverse genomic targets. As it is method development we have analyzed many different iterations and optimizations but we still do not have a manuscript in the time allocated and need to extend it.