Dietary glycosides are abundant in plant foods and undergo extensive biotransformation by the gut microbiota. The resulting aglycones and downstream metabolites often display bioactivities — including immunomodulatory effects — distinct from those of the parent compounds, yet the products formed and the enzymes responsible remain largely uncharacterized. Given that the capacity for these conversions frequently differs between strains of the same species, this project addresses this gap for two abundant human gut commensals, Bacteroides and Prevotella, combining anaerobic culture of a strain panel with untargeted LC-MS metabolomics and comparative genomics to build a strain-resolved map linking substrate, product, and candidate enzyme.