The Enteric Nervous System (ENS) is embedded in the wall of the entire gastrointestinal system and represents the largest part of the peripheral nervous system. Although it is required for basic intestinal functions, such as bowel movement and secretion, it is under-investigated. We have recently categorised the neuronal and glial subtypes of the murine intestine using single-cell RNA-sequencing (scRNA-seq) and found a large heterogeneity (Zeisel et al., Cell 2018; Morarach et al., Nat Neurosci 2021; Li et al., Nat Neurosci 2025). Additionally, through scRNA-seq of embryonic stages, we suggested potential developmental principles underlying the generation of distinct neuronal identities (Morarach et al., Nat. Neurosci. 2021; Li et al., Nat. Neurosci. 2025). We have acknowledged NAISS in these publications. We have recently acknowledged NAISS in a manuscript submitted to PNAS. We would now like to further our understanding of the mechanisms and molecules that regulate the development of the ENS. In recent years, there have been several single-cell transcriptomics and spatial transcriptomics data released, which we would like to integrate and compare with our data. More recently, we have generated new scRNA-seq (including barcoded versions), snRNA-seq, scCUT&Tag, and scATAC-seq datasets for several upcoming publications. Our project proposal has been awarded NBIS/SciLifeLab Bioinformatics Support (WABI) in 2024, and we have been getting continuous support from senior bioinformatician Marcus Ringner. The Computation and storage platform will be used extensively by 3 researchers in the lab. Currently, there are 7 active projects ongoing in the lab that will require continuous access to NAISS. In 2026, we will generate several Xenium datasets from adult mouse colon to investigate neuroimmune interactions, neurogenesis, and neuroplasticity within an inflammatory bowel disease (DSS) model.