This project will use high-resolution spatial transcriptomics and immune receptor profiling to characterize the cellular and clonal organization of human tumors. By integrating 10x Genomics Visium HD data with SpatialVDJ sequencing, we aim to map the spatial distribution, transcriptional states, and clonal expansion of T and B lymphocytes within the tumor microenvironment. The analysis will involve large-scale image processing, alignment, spatial deconvolution, immune repertoire reconstruction, and multimodal data integration across multiple patient samples. The computational workflows require substantial storage and high-performance computing resources due to the size and complexity of Visium HD imaging and sequencing datasets. The project will generate detailed spatial maps of immune-tumor interactions and provide insight into mechanisms of anti-tumor immunity, immune evasion, and therapeutic response.