Using the current computational resources and storage provided, we are currently working on:
• Resolving environmental species complexes and characterizing Shewanella baltica strain groups.
• Investigating Shewanella spp. as a reservoirs of antibiotic resistance genes, including novel variants.
• Metagenomic analyses of pathogen and ARG occurrence in environmental and wastewater samples.
• Advancing our understanding of ARGs dissemination, with particular emphasis on resolving and assembling antibiotic resistance gene-carrying plasmids from Escherichia coli isolates.
• Development of bioinformatic pipelines for bacterial epigenetic studies associated with regulation of gene expression and transfer of antibiotic resistance genes.
We utilize the medium storage to archive all Nanopore sequencing batches generated in-house and to assemble and store our high-quality genome assemblies. This storage also serves as the repository for the databases we employ in our comparative genomics analyses and metagenomic approaches, wich, due to their growing number, require increasingly higher storage capacity. Currently, we are curating more than 200 metagenomes from the Baltic Sea, which significantly will expand the data volume and computational capacities that will be required to handle these datasets effectively.
In summary, the combination of a rapidly expanding dataset and a growing research team, including a current postdoctoral bioinformatic researcher and a master’s student who will work full-time on developing metagenomics pipelines oft he metagenomes described above, is creating an increasingly urgent need for expanded computational capacity.