This project aims to investigate the genomic epidemiology, antimicrobial resistance (AMR), and virulence profiles of Campylobacter spp. and ESBL-producing Escherichia coli isolated from humans, animals, and food sources in Ethiopia. Using whole genome sequencing (WGS) data, we will perform genome assembly, MLST/cgMLST, population structure and phylogenomic analyses, as well as the identification of AMR determinants, ESBL genes, and virulence factors contributing to pathogenicity and transmission. Particular emphasis will be placed on understanding the dissemination of resistance and virulence traits across sectors within a One Health framework. NAISS high-performance computing and storage resources are essential for managing large-scale genomic datasets and enabling computationally intensive analyses.