This project seeks to discover candidate next-generation probiotic strains using a comparative genomics framework. High-quality genomes will be generated from isolated bacteria by combining long- and short-read sequencing approaches. Subsequent functional annotation will identify genes associated with important probiotic characteristics, including host adhesion, metabolic activity, and immune modulation. Comparative analyses across strains will then be used to uncover distinctive and conserved genomic features, enabling the prioritization of promising candidates for future probiotic development.