Interspecific hybridization has been indisputably identified as one of the fundamental evolutionary processes that shapes the evolution of reproductive isolation, speciation, and gene regulation. Addressing the question of how hybridization leads to evolutionary novelty is therefore crucial for advancing our understanding of biodiversity across the tree of life. In the present project, two distinct systems will be utilized: the Italian sparrow, a natural hybrid species with isolated Mediterranean island populations that exhibit divergent hybrid genomes; and synthetic yeast hybrid lines, which will be sampled across a time series following long-term experimental evolution. Multiple sequencing techniques have been employed for the two well-built systems in our laboratory, including whole-genome resequencing and whole/single-cell transcriptomic sequencing. The objective of the data generation process for these two systems is to provide answers to the following research questions: How do hybrid genomes stabilize, and how do evolutionary novelties arise through hybridization?