This project is a merger/continuation of the Runemark Lab’s current storage projects NAISS 2025/6-19 “Hybridization derived novel patterns of gene expression”, 2025/6-14 “Host Plant Driven Speciation in Tephritis conura”, 2025/6-18 “Landscape effects on genetic diversity in wild pollinator species” and 2025/6-21 “The roles of polyploidization and selection in complex floral divergence in Lithophragma bolanderi”. This storage will correspond to compute project NAISS 2025/5-14 “Evolutionary genomics of speciation, hybridization and conservation”, created 2025 on the NAISS recommendation to merge all projects , which corresponded to individual storage projects. We apply for an exemption of the Medium project storage limits as the projects to be merged are allocated 160 000 GiB, with 89,3%, 95%, 90,1% and 99,9% of the respective allocations in use. We will apply for a large storage project in the 2026 round and ask for the exemption until those applications have been evaluated. Scientifically this project addresses the evolutionary genomics of speciation, hybridization and conservation. We lack an understanding of the evolution of regulation of gene expression in hybrids, and its role in hybridization derived novelty. In projects funded by an ERC consolidator grant (Italian sparrows) and VR konsolideringsbidrag (Saccharomyces), I address how hybridization can break up co-inheritance of regulatory elements resulting in transgressive hybrid expression patterns, forming a basis for novel adaptation. In a VR funded project, we address how gene expression interact with coding divergence during ecological speciation, using the peacock fly Tephritis conura which has both host plant specialist- and oligophagous generalist populations enabling studying the roles of plastic and canalized responses along the speciation continuum. In a project funded by FORMAS we use genomics to provide knowledge that can guide spatial planning to maintain genetic variation of butterfly populations, aiming to generalize the knowledge gained as guidelines for the use of genetic data to assess genetic erosion in insect populations. In a VR funded collaboration with Magne Friberg we address how selection interacts with polyploid genomes in shaping genomic architecture and generating novel adaptation. We apply genomics to the ploidy-variable plant-insect interaction model system Lithophragma bolanderi to address how polyploidy interacts with selection to this end. We are investigating if space in current use can be reduced without needing to recreate files. However, additional data from 2 RNA-seq projects (NGI18419, NGI18776), one EM-seq project (NGI18418), one miRNA-seq project (NGI18537) and one nanopore project (NGI18773) and WGS data (NGIP38227)will be delivered shortly. We also have ATAC-seq, further EM-seq and miRNA seq, hi-C and WGS incoming 2026 requiring storage. As one way to reduce storage, Runemark has encouraged collaborators to apply for storage projects. Should these applications be approved (Friberg: NAISS 2026/3-28, Rundlöf: NAISS 2026/3-26) and NGI offers assistance with data transfer to these projects, allocation can be reduced by 30 000 GiB (NAISS 2025/6-18: 20 000 GiB; NAISS 2025/6-21: 10 000 GiB).