Newts possess an extraordinary ability to regenerate their central nervous system (CNS), a capacity that is absent in mammals. Understanding the mechanisms underlying this regenerative ability and determining whether they can be applied to mammals are of great importance. In this project, we will analyze single-cell RNA-seq and bulkRNAseq data from the newt CNS to investigate how newt neural stem cells enter a quiescent state during development, how they are reactivated following neuronal injury, and what cellular and molecular differences exist between newts and mammals in their responses to CNS damage.