Aim: To identify genetic resources (varieties and underlying genes) for reproductive and vegetative traits tolerant to climate change induced drought stress that then can be used for future breeding programs. Background As our global climate changes, organisms are exposed to new abiotic and biotic environments. Plants will experience new pollinator and herbivory communities either due to phenological mismatches or due to shifts in the geographic range of these insects in response to climate change (Outhwaite et al., 2022). Abiotic stress also alters flower development and production with immediate consequences on yield. Temperature and water stress not only reduce flower size and thus pollinator attraction (Kuppler et al., 2021), but also commonly increase pollen sterility which even affects a plants capacity to self-fertilize in the absence of pollinators (Descamps et al., 2018). The loss of yield to pollen sterility is a raising concern in many crops (Pacini and Dolferus, 2019). Finally, in response to these stressors plants, such strawberry plants, that have the ability of clonal reproduction, will opt to invest their resources into asexual reproduction and limit the production of flowers and fruits (Wilk et al., 2009). Crop plants commonly have limited genetic variation as a product of long-term breeding among a limited set of cultivars. Therefore, crops are more susceptible to environmental changes. Crop wild relatives (CWR) are particularly of value in this regard, as new traits of interest can be identified in the CWR and (re)introduced into the crop through breeding programs. The introgression of the day-neutral gene from the wild strawberry F. virginiana into the strawberry cultivar is a great example of the use of wild germplasms to improve strawberry production in producing perpetual flowering varieties irrespective of the season. The woodland strawberry (Fragaria vesca) has also proven itself useful as a CWR for the crop strawberry. Genetic resources mediating resistance against herbivory and florivory have successfully been identified (e.g. Muola et al., 2017; Weber et al., 2020). Particularly beneficial is the fact that its genome has been available for over a decade (Shulaev et al., 2011) and all the varieties explored in this study have been fully sequenced. Furthermore, F. vesca has a vast geographic distribution, spanning all of Europe. Thus, these wild plants are locally adapted to very different climates and local insect communities making it ideal to address questions of climate change. Plant material This section includes a larger collection of 240 varieties of F. vesca covering the latitudinal range of the species in Europe. Phenotyping We have exposed the plant collection to drought stress and measured a serios of vegetative, physiological, floral and reproductive traits. Each genotype was replicated three times within each treatment. Genotyping The genomes of these accessions have been re-sequenced using Illumina NextSeq500, and the mapping of reads on the latest reference genome revealed about 3.5 million SNPs and small indels. Analyses We will carry out population genomic analyses such as GWAS and to search for natural genetic variation contributing to climate adaptation.