NAISS
SUPR
NAISS Projects
SUPR
Mouse Linkage analysis
Dnr:

NAISS 2025/22-1335

Type:

NAISS Small Compute

Principal Investigator:

Wei Meng

Affiliation:

Karolinska Institutet

Start Date:

2025-10-06

End Date:

2026-11-01

Primary Classification:

10610: Bioinformatics and Computational Biology (Methods development to be 10203)

Webpage:

Allocation

Abstract

Clonal fate decisions in the immune system remain poorly understood beyond a tree-like view of lineage differentiation. Here we use single-cell lineage tracing of mouse NK and ILC populations to map clonal relationships and transcriptional states. We uncover continuous clonal fate biases, where anatomical context and clonal composition jointly predict lineage outcomes. Network analysis reveals transcription factor programs shaping these biases, while perturbation experiments show that extrinsic signals can override intrinsic clonal predispositions. Our work provides a framework to dissect lineage specification in innate lymphocytes, with implications for immune development and engineering.