The human gut microbiome is a complex and dynamic ecosystem composed of diverse bacterial species essential for metabolism, immune regulation, and overall host health. To see how human gut microbiome and its function vary with different host conditions, we have different cohorts and some individual studies. In first cohort, we will use 200 individuals (with and without diabetes) with their metadata and microbiome data, with 4 different time points to find relation how their microbiome is impacted with different drugs given to them. In another study, we will look at 4 different good bacteria (considering human gut) at the genome granular level to see more about their functions. And the third study, will be using 25000 pangenomes to make a framework for working more efficiently in metagenomics.