Chromatin exhibits dynamic structural and physical properties which are critical for the regulation of numerous nuclear processes. In our research, we investigate chromatin mobility in the context of DNA damage repair (DDR) in plants. Plant cells are constantly exposed to endogenous and exogenous factors that threaten genome integrity. Yet, DNA is maintained remarkably well, notably against double-strand breaks (DSB) through homologous recombination (HR), in which the damaged strand is to find an intact template for repair. If today HR is relatively well described, much less is known about how chromatin dynamics, particularly chromatin mobility within the nucleus crowded space, is altered in the presence of DNA damage and may impact the repair process. We are currently using quantitative live imaging, DNA-labeling systems (LacO/lacI and ANCHOR), HR reporters, single-molecule RNA labelling methods and molecular genetics to address this question. In Arabidopsis thaliana, we have found that DSBs lead to increased chromatin mobility both locally (at break sites) and globally (across the genome). Taking advantage of NAISS computing and storage systems, we aim to perform a wide range of image analyses, including high-throughput cells and nuclei segmentation, particles tracking, trajectory modelling, detection of nucleolar components, etc