We possess chronic spinal cord scar samples that stem from 7 patients, whose tissues were collected as part of a clinical trial. When extracted, the tissues were immediately fixated and are thus without post-mortem artifacts. We have analyzed the tissues with FFPE-compatible single nuclei RNA sequencing workflow (Chromium, 10x genomics), the data now consisting of approximately 65k cells with a detection depth of 1300 genes per cell. Furthermore, we have used in situ sequencing (ISS) to visualize the spatial expressions of 250 genes on the same tissue sections (now called Xenium, 10x Genomics). Both analyses have recently been completed, and the spatial and single cell data will now be combined, followed by bioinformatic analysis and species comparisons with available datasets. After confirmations and further exploration of scar-features of interest, we intend to functionally test candidates with in vitro/in vivo methods. Hence, we believe this translational map of the spinal cord scar will ensure that relevant information is used for the development and translation of future repair strategies.