The overall goal of our research is to gain a deeper understanding of how the immunological landscape of the human lung influences both health and disease, particularly in the context of lung cancer and respiratory viral infections. This project specifically aims to map immune cell populations, with a focus on natural killer (NK) cells, in distinct lung regions, including the primary bronchus and parenchyma across different lung lobes. We hypothesize that the unique anatomical features of these regions lead to adaptations in the phenotype and function of NK cells and other immune cells, which may vary based on their microenvironmental context. These adaptations are likely to influence the immunological response to malignant cells and viral pathogens in different regions of the lung. By leveraging lung tissue from deceased organ donors, we will construct a comprehensive human lung leukocyte atlas that integrates both protein and transcriptomic data, with a particular emphasis on NK cell subsets and their functional roles. The data will comprise sensitive data since they contain genetic information. This work will not only provide a detailed map of lung immunity but also uncover potential regional variations in immune cell behavior that could inform future therapeutic strategies for lung diseases.