The project deals with genomes and transcriptomic analysis of prokaryotic cells with emphasis on Enterobacterales and/or metagenomes. Bacterial isolates and metagenomes will be sequenced by short read (Illumina) and Long-read (PacBio or Oxford Nanopore). No ethical permission is necessary since the isolates are collected from the environment. If patient isolates are collected in the future, ethical permissions will be obtained.