Escherichia coli enterotoxigenic (ETEC) is a worldwide leading cause of diarrhea, especially in LMIC countries. Multiple colonisation factors have been associated with specific bacterial lineages and predicted to be located on plasmids. However, most of the studies have been done with short-read bacterial assemblies, which are not fully reliable to study plasmids and little is known about them. In our project, we will make use of hybrid assemblies to fully resolve plasmids of 600 ETEC strains around the world to study their genomic epidemiology and understand how the distinct virulence factors are distributed across E. coli population genomics. This would heavily increase our understanding about ETEC population structure and potentialy could throw light to develop novel strategies such CRISPR-based plasmid inactivation or inhibition of conjugation. However, first, an ETEC genomics thorough basic understanding is needed and we aimed to do it with this project.