This project aims to enhance our understanding of chemical ecology-related adaptations in arthropods and their niche-specific survival strategies. The research focuses on identifying chemosensory genes, including ionotropic and gustatory receptors, within Ixodes ricinus and Argiope bruennichi. Additionally, it involves the comprehensive annotation of the Trypodendron lineatum genome to investigate the evolutionary dynamics of chemosensory gene families. Ixodes ricinus, a critical vector for tick-borne diseases in Europe, is the primary subject of study. Despite its public health importance, little is known about its chemosensory system, limiting our understanding of host-seeking behavior and chemical ecology. Through phylogenetic analysis and comparative genomics, this project aims to explore receptor gene conservation and diversification across multiple tick species from five major genera in Ixodidae. The second objective is to investigate the chemosensory adaptations of Argiope bruennichi, an orb-weaving spider species known for its complex mating behavior, including kin recognition, nepotism, and sexual cannibalism. The research focuses on identifying pheromone receptors that play a critical role in these behaviors. Lastly, the project extends to the ambrosia beetle Trypodendron lineatum, whose genome will be annotated and compared with other bark beetles, such as Ips typographus and Dendroctonus ponderosa, to identify gene family expansions associated with their fungal-farming and eusocial lifestyle. To efficiently process and analyze the extensive genomic and transcriptomic data involved, a Snakemake-based bioinformatics pipeline is being developed. This workflow ensures automation, reproducibility, and scalability in sequencing quality control, transcriptome assembly, differential gene expression analysis, and phylogenetic studies. The pipeline is designed to handle multiple species, facilitating a comparative framework for studying the evolution of chemosensory genes in arthropods.