This project aims to investigate the mitogenomes of cnidarians (and additional marine invertebrates) through genome skimming, utilizing a comprehensive approach to assembly, alignment, and subsequent analysis of mitochondrial genomes from approximately 50-100 specimens. By assembling and analyzing mitogenomes from a broad range of species, this study seeks to contribute new insights into the evolutionary biology of hydrozoans, their phylogenetic relationships and taxonomy.
Genome skimming is an efficient method for retrieving mitochondrial and other genomic data from a variety of taxa, and this project will leverage high-throughput sequencing technology to extract mitochondrial DNA from the specimens. The project will involve several computational steps: raw sequencing data will be assembled into mitogenomes, followed by alignment to reference sequences for functional analysis and phylogenetic comparison. Post-assembly cleaning, quality control, and genome annotation will also be key components of the workflow.
This project’s primary objectives are to assemble high-quality mitogenomes for each specimen, conduct comparative genomic analysis, and investigate mitochondrial gene structure. The outcome will contribute valuable genomic resources for this understudied group of organisms, enhancing our understanding of marine invertebrate biodiversity, evolution, and ecology. Additionally, the generated mitogenomes will serve as references for future studies on the phylogeny and systematics of hydrozoans and related taxa.
The computational resources required for this project include computing for the assembly, alignment, and analysis of large sequencing datasets. The use of multiple parallel workflows will facilitate the efficient processing of data from the amount of specimens, ensuring the timely completion of all tasks from initial assembly to final data cleaning and analysis.