Fungal pathogens in the Heterobasidion annosum s.l. genus cause major desctruction in Norway spruce productions forests. A key defence mechanism of the tree is the surround the intruding pathogen with defensive barriers. Understanding the genetics controlling barrier zone formation can aid in breeding spruce trees more resistant to H. annosum s.s.. In this project, we are exploring the genetic regulation of barrier zone formation in the bark and sapwood of Norway spruce. After wounding or inoculation, barrier forming tissue zones were collected at 8-, 20- and 36-days post wounding/inoculation. These samples were RNA sequenced for subsequent differential expression analysis. This data set and analysis aims to provide novel insights into the molecular mechanisms and genetics controlling barrier zone formation in Norway spruce.