The aim of this project is to investigate the association of circulating biomarkers for endothelial dysfunction to the development of kidney disease and cardiovascular disease. Data comes from proteomics performed on blood samples collected at participant inclusion to the two population-based prospective cohorts, namely the Swedish Mammography Cohort (SMC) and the Cohort of Swedish Men (COSM). Both cohorts are part of the national research infrastructure SIMPLER. The endothelial dysfunction markers include angiopoietin-2, thrombomodulin, Tek, von Willebrand factor, plasminogen that together with other potential novel markers from proteomics performed in approximately 12,500 SIMPLER participants. The exposure to endothelial dysfunction markers is tested for association to outcome measures including kidney disease (ICD-code, eGFR from plasma creatinine or cystatin-C, HAVCR1 – kidney injury marker in plasma proteomics), hypertension (blood pressure measurements, anti-hypertensive drugs), myocardial function (diagnosis, NT-proBNP in plasma proteomics), and stroke (diagnosis) during the follow-up time in SIMPLER.