In this project, I aim to process and analyze ancient DNA (aDNA) data from approximately four Homotherium samples to investigate the evolutionary history of this extinct predator. Homotherium was a genus of scimitar-toothed cats with a distinctive morphology that set them apart from modern felids. These large-bodied carnivores, which vanished at the end of the Pleistocene, were characterized by elongated, serrated canine teeth, robust forelimbs, a sloping back, and an enlarged optic bulb—adaptations that likely enhanced their ability to hunt Pleistocene megafauna. The sample set for this study includes one specimen from New Siberian Island, one from the Adycha River in Russia, and two from the Yukon, Canada. In addition to these newly sequenced samples, I will incorporate publicly available data from Homotherium genomes published in Barnett et al. (2020) to expand the dataset and improve comparative analyses. Processing these datasets will involve quality control, read preprocessing, mapping to a reference genome, and downstream analyses such as phylogenetics, population genomics, and demographic reconstruction. The project will enable a comprehensive investigation into the genetic diversity, population structure, and evolutionary history of Homotherium, contributing valuable insights into the factors that shaped the survival and extinction of Pleistocene megafauna.