In this project, we are performing a characterization of pancreatic human tissue using spatial transcriptomics. We have collected tissue biopises from over 20 donors with and without diabetes and are currently mapping single-nuclei rnaseq profiles to the spatial transcriptomics data to investigate the spatial organization of pancreatic tissue during progression to diabetes. We perform this analysis in the setting of T2D, where we identified an inflammatory response in the tissue, and also T1D, where autoimmune attack to pancreatic islets is the main cause. In parallel to this, we are also running a project to find biomarkers of disease severity in another autoimmune disease (sytemic sclerosis, scleroderma) using cell-free RNA. In this project we are also starting to investigate the effect of viral infections in patient response to treatment.