Cellular barcoding involves to label individual cells with unique nucleic acid sequences, termed barcodes. Cellular barcoding can be used to track millions of cells in parallel making it an efficient approach for investigating heterogeneous populations of cells. My lab is using cellular barcoding in combination with single-cell and spatial RNA-seq to a) track the developmental history of the mammalian brain (lineage tracing) and b) to reveal principles of neural network formation and maintenance (circuit tracing). In this project, we aim to use a large number (thousands to millions) of unique genetic barcodes to track the developmental origins of the mammalian brain during normal development and in mouse models of autism. Moreover, we want to understand the rules of neural network assembly during mouse forebrain development using circuit barcoding. We will use single-cell and spatial transcriptomics to read out barcodes and reconstruct lineages/circuits using computational approaches.