This compute project is submitted as a joint project for the Runemark lab, Replacing our three current compute projects and, in addition, encompassing the computational needs for an additional project. The research in the Runemark lab is centered around how novel variation, the raw material for adaptation, arises and is preserved in populations. A major focus is how gene expression and its regulation enables ecological adaptation, and in particular how hybridization induced alterations to gene expression may result in novel adaptation. Another important line of inquiry is how genetic variation is maintained and distributed in landscapes subjected to anthropogenic change. Finally, our work also addresses how large changes to genomic architecture, including polyploidy, enables novel adaptive variation to arise. The three current compute projects held by the Runemark lab that would be replaced by the current project are: NAISS 2024/5-24 used for projects on genetic monitoring of connectivity, diversity and adaptive potential NAISS 2024/5-1 used for projects addressing the role of gene expression in enabling adaptation to a new host plant in Tephritis flies NAISS 2024/5-93 investigating how polyploidization gives rise to novel variation important for interactions with pollinators In addition to those compute projects, we also have a storage project for a NAISS 2024/6-5, for which there is no current compute project. This project addresses the role of hybridization in creating novel variation, and disentangles the regulatory changes that underly the novel gene expression signatures arising in both early and later generation hybrids.