Diabetes can lead to both acute and chronic complications. One such complication, is diabetic foot ulcers where patients suffer from chronic foot wounds. This condition requires frequent hospital visits, antibiotic treatments and surgical interventions. Although treatment of these chronic foot wounds is often not sufficient to prevent lower limb amputation. Monocytes and macrophages are essential mediators in this aspect and contribute significantly to the regulation of wound healing. However, despite their vital role in tissue healing and repair, very little is known about how they might contribute to the dysregulation of wound healing in patients with diabetes. In this project, we aim to characterize the immune response of monocytes and macrophages in diabetic patients with healing and non-healing foot ulcers by using single cell RNA-sequencing and Olink proteomics analysis. Furthermore, functional validation will be performed using an in vitro model of human skin 3D cultures. The results from this study will reveal new insight into the dysregulation of immune functions in diabetic patients and could potentially provide new therapeutic targets for chronic foot wounds.