This computing project will be used to continue analyses of eDNA metabarcoding data in the two projects: 1. Marine biodiversity associated with kelp forests at the Swedish West coast 2. The effect of small-scale marine aquaculture on marine biodiversity. The macroalgae (kelps) are known to provide habitats to many benthic invertebrates and fish species but so far studies on their effect on biodiversity have been limited to particular group of organisms or species. We will use the power of eDNA to describe the whole species communities associated with the kelp forests and in areas outside. Small-scale marine aquacultures, such as marine community gardens ("marina kolonilotter") are suggested to have positive effects through uptake of nutrients, reducing particles in the waters and providing substrate for other species. However, there is no quantitative studies of this effect. We will use eDNA to investigate the impact of small-smale marine aquaculture on biodiversity as part of EU HORIZON project investigating the potential for small-scale aquaculture in Nordic countries. Both projects started in 2024 and will be continued in 2025 - see Activity report. At present there is data for 4 metabarcoding pools that has not been analyzed yet due to the change of my working tasks at GU, and following lack of research time in 2024. This is why the allocated computing resources at Uppmax has been barely used during 2024. Both projects however run through 2025 and the deliveries (a report and a scientific publication) are due by June 2026. The data analyses and more sequences are therefore planned for 2025, and the changes to the time plans have been approved by the project funders. In addition to the computing time I am also applying for a small storage project (proposal NAISS 2024/23-743), for a temporary storage of raw data and temporary files. The existing sequence data at present is stored at disks, waiting for the proposal decisions.