Severe acute respiratory syndrome Coronavirus type 2 (SARScov2) is the cause of the recent COVID-19 pandemic. We performed an experimental Cas13-SARScov2 genome-wide screen to identify gRNAs that would allow Cas13d to degrade the viral RNA. In this project, we generated in-house RNA-seq data with different condition (Wildtype and multiple treatments). At the same time, we will get the sequencing data from previous published article. We need to identified the coverage distribution and targeting efficiency in Cas13d-mediated SARS-CoV-2 RNA decay system using transcriptomics method. What’s more, we will find candidate regions of RNA vulnerability by comparing these effects in different conditions. We will verify the function of top ranked regions and corresponding gRNAs in RKO cells. Finally, we will find potential genes and pathway responsible for RNA decay by transcriptional methods (such as differential gene expression and GO analysis). We expect that this study will determine efficiencies of thousands of small RNAs targeting a viral genome and established a platform that can identify targetable regions of any RNA virus rapidly, it may provide new sights in the clinical research.