The "Soil Metagenomics and Metatranscriptomics Project" at Sveriges Lantbruksuniversitet aims to revolutionize our understanding of agricultural soil ecosystems by identifying genetic markers and active gene expressions associated with pest resistance. This research focuses on high-throughput metagenomic and metatranscriptomic sequencing of soil samples from diverse agricultural settings to uncover microbial communities, their genetic contributions, and their real-time expression profiles related to pest management. By isolating and analyzing DNA and RNA from these samples, the project seeks to discover, catalog, and determine the activity of genes that confer resistance to pests in crops. Insights gained will inform the development of biocontrol strategies and enhance crop resilience, addressing pressing global food security challenges. Utilizing advanced bioinformatics tools, this project will analyze extensive datasets to identify key genetic interactions and active pathways within the soil microbiome. The expected outcomes include a comprehensive database of pest resistance genes, a set of predictive tools for pest management, and an understanding of the microbial functional potential and its expression under agricultural conditions, contributing to sustainable agricultural practices.