This project aims to identify the main members of the microbiota sampled from our artificial fish gut system with various probiotic treatments at different time points and investigate the interactions between the probiotics, gut microbiota, and metabolomics over time. All raw 16S sequencing data will be processed and analyzed by QIIME2 software and R packages on UPPMAX HPC. After combining the sequencing and metabolomics results, statistical tests and models will be done using R packages to understand the relations.