Brugia malayi is one species of roundworm that causes the disease Lymphatic Filariasis, a parasitic disease mainly affecting individuals in the developing world who lack access to antibiotics and live in conditions of poor hygiene. The lifecycle of B. malayi occurs partly in a mosquito host with the development of infectious larvae from microfilariae and occurs partly in a human host with the development of larvae into adult worms and the release microfilariae into the bloodstream. To understand more about the infectious worm B. malayi , the spatial gene expression pattern of the ovary tissue of the female worm will be constructed using the Spatial Transcriptomics method.