The stem cells in the developing central nervous system (CNS) give rise to different nerons and glia, under specific genetic regulations. However, such stem cell potential is largely lost in the adult CNS, making it difficult to regenerate after injuries. Recent studies show stem and progenitor cells in the adult CNS can re-enter a active phase, recapitulates fetal stem cells during neurodevelopment and give rise to multiple cell lineages. This potential of stem cells provide an attractive possibility for regenerative medicine. However, how different between fetal neural stem cells and adult stem cells are still largely under studied. To further understand this question for better gene and cell therapies for CNS injuries, we used multiomics to compare rodent CNS development and regeneration after injuries. We will analyze the different genetic regulations of CNS cell types under these conditions, and provide better genetic targets to manipulate cell fates of the neural stem cells. Thus, this project will give new targets on genetic control of stem cells and shed light on the development of new therapies for CNS injured patients in the future.