The capture of atmospheric CO2 by photosynthesis and its potential storage via soil C sequestration (SCS) in agricultural lands is an important, cost-effective negative carbon emissions technology for mitigating climate change. SCS also provides ecosystem services to society such as increased soil quality and fertility. There is, however, a need to quantify SCS rates that can be realized in Sweden. This project addresses the effect of management practices (MPs) including crop types, crop rotations and cover crops on SCS. The impact of these MPs is assessed at regional and national level, considering differences in farming systems and the interactions of SCS with climate change. To achieve these objectives, we will synthesize and develop new empirical knowledge from long-term field experiments and spatio-temporal soil inventories, and integrate this data with novel modelling concepts to predict SCS. These models will present an advanced understanding of processes regulating SCS, and provide realistic SCS rates and associated uncertainties under Swedish conditions. Outcomes from the project will be applicable for guiding policies, and for improving extension service tools at the farm scale. The project team includes world-leading scientists, and a multi-stakeholder advisory panel will ensure the involvement and expertise from the public and agri-business sectors for constructing representative scenarios of changes in MPs promoting SCS.