Cystic fibrosis (CF) is a genetic disease characterised by chronic airway infections and loss of lung function. Around 30% of individuals with CF develop a CF-related diabetes. Diabetes is associated with poor lung function, but the underlying mechanisms are not fully understood. In this project, we want to investigate if there are differences in the airway microbiome between CF patients with and without diabetes. We have collected sputum samples from approximately 55 patients, of which 1/3 has CF-related diabetes. The microbiomes of the samples will be analysed using 16s sequencing and the microbiomes will then be compared between individuals with and without diabetes with regard to alpha and beta diversity and enrichment analyses et c.