Application of metagenomic methods on ancient DNA sequences has extended our knowledge of the lifestyle of historical populations. However, current metagenomic pipelines are designed to work with the sequencing data of modern samples. Thus, these protocols should be tailored to accommodate the challenges in ancient DNA sequences. Previously, National Bioinformatics Infrastructure Sweden (NBIS), Wallenberg Advanced Bioinformatics Infrastructure (WABI) supported our project and our crew grew bigger. With this significant support, we continued building the metagenomic analysis workflow to understand the metagenomic composition in ancient DNA libraries. The workflow is now finished and available online. We now have several ongoing projects where we want to utilise the workflow and I would like to apply for a small-sized compute project to run screening and alignment of new data.