Non-small cell lung cancer (NSCLC) is the most common cause of cancer related death in Sweden. Despite improvements in therapy the prognosis remains poor, and the molecular mechanisms of tumorigenesis are only fragmentarily understood. In this project we have generated whole-exome sequencing data from patients with NSCLC with sequencing results from the primary tumor, patient derived organoids and cell lines derived from the same tumor as well as matched normal tissue. We will analyze the data using the Sarek pipeline built in Nextflow to generate small variants and copy number aberrations.