Dissection of synaptic proteomic spatial distributions

NAISS 2024/5-149


NAISS Medium Compute

Principal Investigator:

Erik Fransén


Kungliga Tekniska högskolan

Start Date:


End Date:


Primary Classification:

30105: Neurosciences



The project is a collaboration between Erik Fransén and Martin Rehn at CST, EECS, KTH and Seth Grant at University of Edinburgh Center for Clinical Brain Sciences from which we obtain data on synaptic protein abundance in the brain at single synapse resolution. We have collected data on protein expression over the entire mouse brain (Zhu F, Cizeron M, Qiu Z, Benavides-Piccione R, Kopanitsa MV, Skene NG, Koniaris B, DeFelipe J, Fransén E, Komiyama NH, Grant SGN. Architecture of the Mouse Brain Synaptome. Neuron. 2018 Aug 22;99(4):781-799.e10. Doi: 10.1016/j.neuron.2018.07.007.) and (Cizeron M, Qiu Z, Koniaris B, Gokhale R, Komiyama N, Fransén E, Grant S. A brainwide atlas of synapses across the mouse life span Science Vol. 369, Issue 6501, pp. 270-275, 2020. DOI: 10.1126/science.aba3163). The data is containing 50+ brain sections each containing 100 million+ synapses. Pilot studies have shown a large regional variability (Erik Fransén. Synaptic heterogeneity in the brain at single synapse resolution. Emerging Topics in Artificial Intelligence (ETAI) 2021 11804, 118040K, 2021; E. A. FRANSÉN, M. REHN, Z. QIU, M. J. CIZERON, B. KONIARIS, S. G. N. GRANT. Synapse population distributions reveal synaptome architecture including regional differentiation. Society for Neuroscience Annual Meeting, 369.01, 2019).