Neuroblastoma (NB) is a pediatric neuroendocrine tumor of the peripheral nervous system which usually manifests in the adrenal gland, abdomen, and chest. The origin of NB is thought to be neural crest cells (NCC) and its derivative sympathoadrenal (SA) cells. To study NB development, we reprogrammed non-cancerous fibroblasts from NB patients from healthy individuals into induced pluripotent stem (iPS) cells. The iPS technology allows somatic cells to be reprogrammed to a pluripotent stem cell with the ability to self-renew and to differentiate to almost all human cell types. We successfully differentiate the iPS to NCCs and to SA lineages cells and sent for bulk RNAseq. Data were obtained from the sequencing company and analysis is ongoing. Later on, the scMultiomics will be performed. Our aim is to figure the important timepoint and cell type for NB formation