Cancer, a significant global health issue and a major cause of mortality, exhibits notable diversity arising from various initiating events and evolutionary paths. This diversity spans genetic, transcriptomic, and proteomic aspects, posing challenges to treatment efficacy and facilitating recurrence and cancer spread by promoting metastasis. Current therapeutic approaches, formulated on the premise of uniform responses despite acknowledging inherent diversity, encounter constraints in effectively addressing the heterogeneous nature of tumors. In this study, I aim to elucidate the dynamic functions of ribosomal proteins and their potential impact on cancer cell heterogeneity. Continued investigations, including my recent findings, challenge conventional perspectives on ribosomal proteins, revealing their spectrum of specialized functions, encompassing mRNA translation, cytoskeletal dynamics, and the regulation of cancer-specific genes (once-ribosomes). Preliminary examinations identified a specific protein isoform of ribosomal protein expressed across multiple cancer types, including gliomas. Building on these insights, our research seeks to unveil the distinctive expression patterns of ribosomal proteins in multiple cancer types and a murine glioblastoma model.