Plant mating is a fundamental ecological process governed by pollen production, attraction of animal pollinators, pollen transport by animal pollinators, pollen deposition on stigmas, and successful ovule fertilization. Thus, the characteristics of animal pollinators (body morphology, foraging behaviour, community composition) can determine the rate of self- versus cross- fertilization, influence the incidence of multiple paternity at the fruit and plant levels, and impose constraints on the ecology and evolution of plant mating systems. This research project will harness the power of Next-Generation Sequencing to evaluate how the characteristics of animal pollinators (body morphology, foraging behaviour, community composition) drive the ecology and evolution of plant mating system. Thus, we address a fundamental challenge in the study of plant-pollinator interactions: to connect the dynamics and complexities of the pollen transport mechanism with the outcomes of plant mating. This research project is centred on Viscaria vulgaris (Caryophyllaceae), a perennial plant that interacts with a wide diversity of animal pollinators. Broadly, our methodology is based on an observational field survey across Scania in southern Sweden and on an experimental approach that excluded pollinator functional groups. We will adopt a Restriction Site Associated DNA Sequencing (RAD-Seq) approach that will allow us to assess Single Nucleotide Polymorphisms (SNP) and estimate the rate of self- versus cross-fertilization and the incidence of multiple paternity at the fruit and plant levels. Together, these approaches will allow us to assess how particular functional groups of pollinators impose constraints on the ecology and evolution of plant mating systems. This research project will yield several publications that will be of general interest for the study of plant-pollinator interactions.