Several tissues in the human body are able to regenerate after having suffered from an injury. Unfortunately, this is not the case for the Central Nervous System (CNS). In response to CNS injuries, resident cell types proliferate to generate a scar. Scar formation prevents secondary damage and promotes wound repair, but it is nevertheless insufficient for functional repair. Multiple cell types contribute to scar formation, including resident neural stem cells, astrocytes and perivascular cells. However, given that the cellular compartments contributing to scar formation have been studied using cell type-specific fate mapping, we lack an unbiased picture of all scar forming cells, their molecular identity and how they are generated over time. In this project we will use a division-coupled single cell sequencing approach that does not rely on cell type labeling but on a common property of scar forming cells: cell proliferation. Using this strategy we will characterize scar formation over time in mouse models of spinal cord injury. This could be used in the future as a reference map for scar formation and enable the specific targeting of the described cell types and states.