Probing and programming the spatiotemporal dynamics of proteins within neuronal circuits

NAISS 2023/5-576


NAISS Medium Compute

Principal Investigator:

Onur Dagliyan


Karolinska Institutet

Start Date:


End Date:


Primary Classification:

10203: Bioinformatics (Computational Biology) (applications to be 10610)



We are currently studying the spatiotemporal dynamics of a set of proteins that transmit the signal from synapses to the nucleus by using a broad spectrum of techniques including protein engineering, imaging, biochemistry, electrophysiology, genomics, transcriptomics, and proteomics. By focusing on experience-driven neuronal circuit responses at the molecular level, we aim to develop new therapeutic approaches for neurodevelopmental disorders such as autism spectrum disorders. To reconstruct the protein networks, we are studying activity-dependent RNA binding protein mediated transcript regulation using CLIP-Seq (crosslinking immunoprecipitation sequencing and LACE-Seq (linear amplification of complementary DNA ends and sequencing, which maps the RBP’s interacting RNAs' and regulated networks in vivo. Further, we will perform cell type specific spatial transcriptomics using the Vizgen´s MerScope platform, and potential translatomic reprogramming will be studied using Ribo-tag-sequencing. We will be using Cut&Run Sequencing to determine genomic binding of our target proteins.