In this project, we are going to develop a pipeline for automatically assembling and binning the raw metagenomics data and generate the enzyme constraint Genome-Scale Metabolic Models (ecGEMs). The simulation of the ecGEMs will also be performed in this project. The published metagenomics data from human gut and oral will be collected from the online databases and used to test the pipeline. Thereafter, the open source raw metagenomics data from human gut of liver cancer patients will be analysed by the pipeline. EcGEMs for each microbe in the samples will be generated and simulated for better understandings of the relationship between liver cancer and the gut microbial community.