Molecular characterization of B cell activation
Dnr:
NAISS 2023/22-1314
Type:
NAISS Small Compute
Principal Investigator:
Taras Kreslavsky
Affiliation:
Karolinska Institutet
Start Date:
2024-01-01
End Date:
2025-01-01
Primary Classification:
30110: Immunology in the medical area
Webpage:
Allocation
- Klemming at PDC: 500 GiB
- Crex 1 at UPPMAX: 128 GiB
- Dardel at PDC: 1 x 1000 core-h/month
- Rackham at UPPMAX: 1 x 1000 core-h/month
Abstract
The purpose of this project to dissect B cell activation by a combination of single cell and bulk RNA-seq. Murine B cells were activated in vivo, sorted and subjected to single cell and bulk RNA-seq as well as scATAC-seq. For single cell RNA-seq sorted cells were subjected 10x genomics-based library preparation and Illumina sequencing.