Recent archaeological excavations in the courtyard of the castle of Ponferrada, in Spain, have unearthed two mass graves. In face of this, this thesis will involve genetic and computational analysis to help to answer some of the intriguing questions regarding these human remains (Are they relatives to each other? What is their genetic geographical origin? Are they locals? - one hypothesis is that they are Napoleonic soldiers - Do they present any microbial evidence that could be possible linked to their death?). The aims of this study are to establish the: biological sex, (ii) kinship, (iii) phenotype (e.g., skin, eye, and hair color), (iv) biogeographic ancestry, and (v) infection history (metagenomic analysis). Depending on the time available and the quality of the genetic data obtained, genotype imputation might be performed to increase genome coverage. To do this investigation, DNA will be extracted from the teeth of human remains that we are allowed to sample following Dabney et al. (2019) protocol. After, library preparation and whole genome sequencing will be performed at the National Genomics Infrastructure (NGI). Computational analysis will be performed using the UPPMAX system to derive the information that might answer our research questions, previously mentioned.