Parkinson’s disease (PD) is a progressive neurodegenerative disorder, which critically results in the loss of midbrain dopaminergic (mDA) neurons. Current treatments for PD are symptomatic, but cell replacement therapies such as transplantation of midbrain dopaminergic (mDA) progenitors or reprogramming of glial cells into mDA neurons, have been identified as interesting therapeutic strategies. While cell transplantation is currently under clinical trials, reprogramming strategies of midbrain astrocyte remain at an experimental stage and require further development. In this project, we are aiming to conduct large-scale integration of publicly available datasets to define adult human midbrain astrocyte subtypes and further characterize a specific cell population for cell conversion methods.