Feralization and single cell sequencing studies

NAISS 2023/5-474


NAISS Medium Compute

Principal Investigator:

Dominic Wright


Linköpings universitet

Start Date:


End Date:


Primary Classification:

10609: Genetics (medical to be 30107 and agricultural to be 40402)



This project is based on the data for my ERC consolidator project that examines the effect of feralisation on the methylome, genome and transcriptome of the chicken. We have a project currently (SNIC 2022/5-577). We currently have 15X coverage from over 500 chickens, plus transcriptomes from a further 1.5k birds also now uploaded (with the SciLife lab used for data generation in several instances). We will now add a further 400 methylomes in 2024. This data is being actively analysed, however due to two post-docs leaving in 2023, the analysis was limited for the middle-end of 2023. I am now hiring two additional bioinformaticians for 2024. In addition, we have also generated a massive amount of single cell Multiome data this year (combined RNA and ATAC seq data). This data consists of 6 S4 novogene flowcells (60billion reads), and analysis is also ongoing on this project. Currently, we have clustered the data, performed eQTL mapping I am now seeking to extend the useage for this project for another year.